Mangul and his team used simulated data to test the feasibility of using RNA-Seq to study the adaptive immune repertoire. ImReP is able to identify 99% CDR3-derived reads from the RNA-Seq mixture, suggesting it is a powerful tool for profiling RNA-Seq samples of immune-related tissues.
to mine the functional nanobody repertoire from a phage-displayed camelid immune library directed to the recepteur d’origine nantais (RON) receptor kinase. Challenges to this application of NGS include accurate removal of read errors, correct identification of related sequences, and establishing meaningful inclusion criteria for sequences-of-
Aug 31, 2016 · High-throughput sequencing has been a breakthrough technology for the study of the immune repertoire and has already had a profound effect on our knowledge of the immune systems physiology during health and disease. In particular, HTS has transformed our understanding of immune repertoire formation during infection, malignancy, and autoimmunity. Immunosequencing: applications of immune repertoire deep sequencing. Curr Opin Immunol. 2013; 25(5):646-52 (ISSN: 1879-0372) Robins H. Advances in high-throughput sequencing have enabled the development of a powerful new technology for probing the adaptive immune system.
Immune repertoire deep sequencing has made it possible to comprehensively profile the TCRs of a lymphocyte population and has been widely applied to TILs . The technology has enabled novel approaches for diagnosing and prognosticating diseases with a driving immune component by identifying repertoire patterns associated with May 20, 2019 · The platform is an end-to-end solution for immuno- and precision oncology biomarker discovery applications, simultaneously enabling the analysis of: tumor escape mechanisms (including HLA typing and somatic mutation detection), immune repertoire profiles, neoantigen load, tumor mutational burden (TMB), microsatellite instability (MSI ... Feb 22, 2017 · Atreca Presents New Preclinical Findings For Novel Cancer Immunotherapy Platform At Molecular Medicine Tri-Conference 2017 - read this article along with other careers information, tips and advice on BioSpace